Colonization of wheat roots by an exopolysaccharide-producing Pantoea agglomerans strain and its effect on rhizosphere soil aggregation. Critical protective role of bacterial superoxide dismutase in rhizobium-legume symbiosis. Wild type Pantoea sp. The carotenoid biosynthesis pathway has been well characterized in Pantoea and consists of six enzymes: geranylgeranyl diphosphate (GGPP) synthase CrtE, phytoene synthase CrtB, phytoene desaturase CrtI, lycopene cyclase CrtY, β-carotene hydroxylase CrtZ, and the zeaxanthin glucosyltransferase CrtX (To et al., 1994; Sedkova et al., 2005). Alqueres, S., Meneses, C., Rouws, L., Rothballer, M., Baldani, I., Schmid, M., et al. Plant Dis. Sequences from Populus rhizosphere isolates GM01, YR343 (Brown et al., 2012) and three other unidentified isolates for which data was available from genome sequencing efforts on IMG were aligned using the Translation alignment tool within GeneiousTM (v6.0.4-www.geneious.com) with 94 total representatives of Pantoea and related taxa of Erwinia, Tatumella and other Enterobacteriaceae for further phylogenetic analysis with Chronobacter sakazakii as an outgroup. YR343 is a motile, rod-shaped bacterium isolated from the roots of Populus deltoides that possesses the ability to solubilize phosphate and produce the phytohormone indole-3-acetic acid (IAA). Biol. Tang, Y. W., and Bonner, J. Briefly, 500 μl of overnight cultures was diluted into 50 ml of M9 minimal medium plus L-Tryptophan (200 μg ml-1 final concentration), and incubated overnight at 28°C. Due to the observation that the ΔcrtB mutant is impaired in biofilm formation and IAA production, we speculated that this mutant may also be affected in root colonization. Comparisons of growth in liquid cultures between wild type and the ΔcrtB mutant showed that the growth rates were very similar when cells were grown in LB or R2A medium (data not shown), but that the ΔcrtB mutant reached stationary phase at a lower cell density compared to wild type cells when grown in M9 minimal medium (Figure 5A). Egamberdiyeva, D., and Höflich, G. (2001). Mol. For soil samples, 1 g of soil was mixed with 4 ml of PBS, then vortexed and plated similarly. Microbiol. Tarifs Rentrée 2012. (2008). Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis. Singlet oxygen quenching ability of naturally occurring carotenoids. Colonies lacking yellow pigmentation were screened by PCR to verify that the crtB gene was deleted. YR343. Mol. 52, 195–204. gypsophilae. Métier. The production of indoles was measured using a colorimetric assay. Acta 352, 361–370. Examination of the plant roots indicated that there were significantly more wild type cells (green) than mutant cells (red) attached to the roots (Figure 6D). YR343 is shown by a zone of clearing surrounding the colony. It is likely that the Raman intensities of the carotenoid bands are pre-resonantly enhanced, which is a Raman scattering process that occurs when the frequency of the excitation laser beam lies just below the frequency of an electronic transition of the chromophore in the irradiated molecule. (1991). Annu. Intégrer l'école' Lycée Rouvière ? doi: 10.1111/j.1751-1097.1991.tb01989.x, Kamilova, F., Kravchenko, L. V., Shaposhnikov, A. I., Azarova, T., Makarova, N., and Lugtenberg, B. 153, 205–212. doi: 10.1128/mBio.01251-1215, Kulkarni, G. B., Nayak, A. S., Sajjan, S. S., Oblesha, A., and Karegoudar, T. B. YR343 and ΔcrtB in M9 minimal medium. Mixtures of wild type and the ΔcrtB mutant were distinguished by colony color (wild type colonies were yellow, while the mutant colonies were white). (2009). Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. Effect of carotenoid overproduction on oxygen tolerance of nitrogen fixation in Azospirillum brasilense Sp7. Biological role of pigment production for the bacterial phytopathogen Pantoea stewartii subsp. Regulation of membrane lipid fluidity in Acholeplasma laidlawii: effect of carotenoid pigment content. 25, 765–778. Swarming motililty has been associated with virulence in the plant pathogen, P. stewartii, which colonizes the plant xylem, blocking flow and causing wilting in its plant hosts, including corn (Herrera et al., 2008). YR343 could produce IAA, which is synthesized from the amino acid tryptophan (Patten and Glick, 1996). doi: 10.1590/S1415-47572012000600020, Brady, C. L., Cleenwerck, I., van der Westhuizen, L., Venter, S. N., Coutinho, T. A., and De Vos, P. (2012). YR343 treated with different concentrations of hydrogen peroxide shows a decrease in peak intensity at 1500 cm-1 upon treatment with hydrogen peroxide. (1987). YR343. Bacterial biosynthesis of indole-3-acetic acid. J. Biotechnol. Plants were grown for 21 days at 24°C with a 12 h light and 12 h dark photoperiod. doi: 10.1094/Mpmi.1918.104.22.1684, Merlin, J. C. (1985). Infect. YR343 on such characteristics as stem height, leaf number and size, and root area. Britton, G. (1989). 7, 617–635. Thus, we hypothesize that changes in membrane organization as a consequence of the loss of carotenoids could impact the function of membrane proteins involved in signaling and transport, and result in the observed defects in IAA production and biofilm formation in Pantoea sp. These peaks are reduced in the ΔcrtB mutant. This bacterial strain is referred to as Pantoea sp. YR343 (left) and ΔcrtB (right) and grown for 48 h showing loss of pigmentation in the mutant strain. Shoot tips were then washed three times in sterile water before inoculation into MS medium (per 1 l of medium: 4.43 g MS salts, 0.5 g MES hydrate, 30 g sucrose, 5 g activated charcoal, 1.5 g Gelrite and 1 ml plant preservative mixture (PPM). Biochem. Plant Mol. Nutr. GM01; Brown et al., 2012) within a monophyletic Pantoea group (90%) and forms a unique, well supported (100%) group, basal to the recently described P. rwandensis and P. rodasii, that are known to form lesions on leaves of plantation-grown Eucalyptus trees (Brady et al., 2012) (Figure 1). doi: 10.1007/BF00010355, Sandmann, G., and Misawa, N. (1992). Biol. Swimming and swarming motility was examined on LB containing 0.3% w/v agar or 0.6% w/v agar supplemented with 0.4% w/v glucose or 0.4% v/v glycerol, based on previous studies (Herrera et al., 2008). Liaaen-Jensen, S., and Andrewes, A. G. (1972). J. Syst. Rhizosphere bacterial signalling: a love parade beneath our feet. YR343 is not pathogenic to P. deltoides WV94. YR343 also allowed us to follow the effect of H2O2 on carotenoids. While the catalytic proteins, encoded by bscA and bscB, are conserved in both types of cellulose synthesis gene clusters, there are genes unique to each operon for which the functions are not well known (Romling, 2002). Sterilized seeds were soaked in double distilled H2O containing 0.1% w/v agar at 4°C for 4 days and then germinated on Murashige and Skoog (MS) agar containing 0.25% w/v sucrose (Murashige and Skoog, 1962). Acceptez-vous de recevoir des notifications d'orientation ? FIGURE 5. Appl. Functional microdomains in bacterial membranes. stewartii. 60, 579–598. To compare growth rates of Pantoea sp. FIGURE 3. P. rwandensis, P. rodasii, P. vagans, and P. eucalypti have been implicated as the causal agents of bacterial blight, leaf lesions, and dieback in eucalyptus (Coutinho et al., 2002; Brady et al., 2009, 2012). doi: 10.1094/Pdis.2002.86.1.20, De Maayer, P., Chan, W. Y., Venter, S. N., Toth, I. K., Birch, P. R. J., Joubert, F., et al. (1994). We also analyzed motility behavior in this organism using LB medium containing either 0.3% (swimming) or 0.6% (swarming) agar (Figure 2E). The presence of cellulose in Pantoea sp. R. Soc. (B) LB plates streaked with wild type Pantoea sp. YR343 to establish a strong interaction with the plant root, perhaps due to defects in IAA production or in biofilm production. (2010). Biophys. Adv. Genetic organization of the cellulose synthase operon in Acetobacter xylinum. YR343 does not have a complete tryptamine pathway. 71, 8141–8146. Finally, the loss of carotenoids may affect the ability of Pantoea sp. We would also like to acknowledge Justin Jagodinski for his preliminary work characterizing the ΔcrtB mutant. Imaging of root colonization was performed by staining root tissue with 5 μM Syto61 (Life Technologies) to stain all bacterial cells that were attached to the root. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. 75, 1143–1150. Pseudomonas fluorescens induces strain-dependent and strain-independent host plant responses in defense networks, primary metabolism, photosynthesis, and fitness. Funct. doi: 10.1007/s11104-008-9568-6, Robert, B. stewartii exhibits surface motility, which is a critical aspect of Stewart’s wilt disease development on maize. Amellal, N., Burtin, G., Bartoli, F., and Heulin, T. (1998). An overnight culture of Pantoea sp. Photosynth. Briefly, roots were weighed, washed by vortexing at least 30 s with 3 ml of PBS and a few small glass beads and dilutions of the wash solution was plated on R2A to measure colony forming units (CFU). The wild type strain shows a spectra dominated by peaks (highlighted by arrows) corresponding to zeaxanthin. Soc. Resonance Raman spectroscopy of carotenoids and carotenoid-containing systems. doi: 10.1016/s0304-4173(87)80008–80003, Cogdell, R. J., Howard, T. D., Bittl, R., Schlodder, E., Geisenheimer, I., and Lubitz, W. (2000). YR343 is most closely related to Pantoea sp. J. Raman Spectrosc. nov. Int. YR343 on Populus deltoides WV94 growth. This motile, rod-shaped bacterium is able to solubilize phosphate and produce IAA. Impact Factor 4.235 | CiteScore 6.4More on impact ›, Max Planck Institute for Plant Breeding Research, Germany, Austrian Institute of Technology (AIT), Austria, Ludwig Maximilian University of Munich, Germany. YR343. Genomic comparisons of the carotenoid biosynthesis operon in Pantoea sp. Afterward, seeds were washed briefly in 70% ethanol and rinsed several times in sterile water. 86, 106–111. YR343 on cultured poplar cuttings. Acad. Organic acids, sugars, and L-tryptophane in exudates of vegetables growing on stonewool and their effects on activities of rhizosphere bacteria. 11, 634–642. That the ability to overcome oxidative stress is important for root colonization was shown in Gluconoacetobacter diazotrophicus PAL5 by demonstrating that production of superoxide dismutase and glutathione reductase were essential for colonization of rice roots (Alqueres et al., 2013). YR343. doi: 10.1098/rstb.2000.0696, Coutinho, T. A., Preisig, O., Mergaert, J., Cnockaert, M. C., Riedel, K. H., Swings, J., et al. nov., Pantoea deleyi sp. Chamberlain, N. R., Mehrtens, B. G., Xiong, Z., Kapral, F. A., Boardman, J. L., and Rearick, J. I. (2013). Agron. doi: 10.1016/S0378-1119(96)00423-4, Dussault, D., Caillet, S., Le Tien, C., and Lacroix, M. (2008). YR343 (data not shown). 69, 253–257. Copyright © 2016 Bible, Fletcher, Pelletier, Schadt, Jawdy, Weston, Engle, Tschaplinski, Masyuko, Polisetti, Bohn, Coutinho, Doktycz and Morrell-Falvey. Examination of Enterobacteriaceae genomes shows the existence of two distinct cellulose synthesis gene clusters, represented by the operon structure found in Gluconacetobacter xylinus (Wong et al., 1990) compared to the operon structure found in Escherichia coli (Zogaj et al., 2001). A new leaf blotch disease of sudangrass caused by Pantoea ananatis and Pantoea stewartii. YR343. Strains of P. agglomerans have also been shown to promote plant growth in wheat, rice, and cotton (Ruppel et al., 1992; Amellal et al., 1998; Egamberdiyeva and Höflich, 2001; Verma et al., 2001; Feng et al., 2006). Appl. Phytopathology 100, 1330–1339. Received: 19 January 2016; Accepted: 24 March 2016;Published: 18 April 2016. Afterward, germinated seedlings were added to each tube. YR343-GFP and the ΔcrtB mutant were normalized to the same optical density (OD600). Mol Plant Microbe Interact 25, 139–150. Three plants were measured for colonization per treatment: uninoculated control, wild type only, ΔcrtB only, and a 1:1 mix of wild type and ΔcrtB. YR343 in this paper. Ann. (E) Sensitivity of wild type and ΔcrtB mutant cells to increasing concentrations of hydrogen peroxide. An overnight culture was diluted 1:100 into either LB, R2A, or M9 medium supplemented with 0.4% w/v glucose and grown statically in a 96-well plate covered with breatheable tape in place of the lid (Breathe-EASIER, Diversified Biotech) at 28°C for 72 h. After 72 h, adherent cells were stained with 0.1% w/v crystal violet stain, then the crystal violet associated with biofilms was dissolved using a modified solution which contained 10% w/v SDS dissolved in 80% v/v ethanol (Tram et al., 2013). Gene 179, 45–51.
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